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Current possibillities of laboratory diagnostics of EBV
Janů, Denisa
;
Janďourek, Ondřej
(advisor) ;
Konečná, Klára
(referee)
Charles university Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study programme: Bioanalytical laboratory diagnostics in health care Author: Bc. Denisa Janů Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: Current possibillities of laboratory diagnostics of EBV Background: The aim of this diploma thesis was to get acquainted with the issue of Epstein-Barr virus infection and its laboratory diagnostics. Other aim was to divide the patients into groups according to the serological results, then evaluate the prevalence of EBV and evaluate the incidence of primary EBV infection in the tested group of patients for the years 2019-2022. Methods: Chemiluminescence immunoassay and indirect immunofluorescence were used to determine specific antibodies against EBV. In some cases, the examination was completed by determining the avidity of anti-VCA IgG antibodies and the microblot array method. Real-time PCR was used to detect EBV DNA. Microsoft Excel 2016 was used to process data and to create tables and graphs. Results: For the years 2019-2022, 14,736 patients were tested for the presence of specific antibodies against EBV in the infectious serology and virology laboratory of Vidia-Diagnostika, 263 patients for the presence of heterophile antibodies...
Detailed record
Diagnostic examination of Epstein-Barr virus in routine laboratory practice
MILÉŘOVÁ, Lenka
The bachelor's thesis summarizes the existing knowledge about the Epstein-Barr virus. In the practical part it focuses on the laboratory determination of antibodies against viral antigens. The results of the laboratory analysis are used to evaluate the viral penetration of the tested group. the results are compared with data reported in the current literature.
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